Coding

Part:BBa_K1813004

Designed by: UBC iGEM 2015   Group: iGEM15_British_Columbia   (2015-07-27)

CYP6G1

CYP6G1

CYP6G1 is a cytochrome p450 enzyme found in the common fruit fly, Drosophila melanogaster. Able to efficiently Hydroxylate imidacloprid at the 4th and 5th carbons, it is capable of bestowing protective properties to organisms that can express it.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 934
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 943


Background of CYP6G1

As an enzyme capable of hydroxylating the 4 and 5 carbons of imidacloprid, it is capable of bestowing a significant protective effect to insects harboring this gene. It has an affinity to 6OH imidacloprid, allowing it to gnerate 4,5 OH imidacloprid [1], which has an ld50 of over 1 microgram vs 57 nanograms for imidacloprid [2]. Used in conjunction with CYP6CM1vQ, (BBa_K1813003) it can be extremely effective in bestowing imidacloprid resistance to an organism.
This coding sequence was synthesized and is codon optimized for E. coli. It was originally identified in Drosophila melanogaster, the common fruitfly [1]. This part is also used in BBa_K1613003 and BBa_K1813012


References

[1] Joußen, Nicole, et al. "Metabolism of imidacloprid and DDT by P450 CYP6G1 expressed in cell cultures of Nicotiana tabacum suggests detoxification of these insecticides in Cyp6g1‐overexpressing strains of Drosophila melanogaster, leading to resistance." Pest management science 64.1 (2008): 65-73.

[2]Suchail, S., Guez, D., & Belzunces, L. P. (2001). Discrepancy between acute and chronic toxicity induced by imidacloprid and its metabolites in Apis mellifera. Environmental toxicology and chemistry, 20(11), 2482-2486.

[edit]
Categories
Parameters
None